The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.
Identifieur interne : 000014 ( Main/Exploration ); précédent : 000013; suivant : 000015The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.
Auteurs : Alexandra Seguin [États-Unis] ; Xuan Jia [États-Unis] ; Aubree M. Earl [États-Unis] ; Liangtao Li [États-Unis] ; Jared Wallace [États-Unis] ; Andong Qiu [États-Unis] ; Thomas Bradley [États-Unis] ; Rishna Shrestha [États-Unis] ; Marie-Bérengère Troadec [France] ; Matt Hockin [États-Unis] ; Simon Titen [États-Unis] ; Dave E. Warner [États-Unis] ; P Tom Dowdle [États-Unis] ; Martin E. Wohlfahrt [États-Unis] ; Elaine Hillas [États-Unis] ; Matthew A. Firpo [États-Unis] ; John D. Phillips [États-Unis] ; Jerry Kaplan [États-Unis] ; Barry H. Paw [États-Unis] ; Jonathan Barasch [États-Unis] ; Diane M. Ward [États-Unis]Source :
- The Journal of biological chemistry [ 1083-351X ] ; 2020.
Abstract
Mitochondrial iron import is essential for iron-sulfur cluster formation and heme biosynthesis. Two nuclear-encoded vertebrate mitochondrial high-affinity iron importers, mitoferrin1 (Mfrn1) and Mfrn2, have been identified in mammals. In mice, the gene encoding Mfrn1, solute carrier family 25 member 37 (Slc25a37), is highly expressed in sites of erythropoiesis, and whole-body Slc25a37 deletion leads to lethality. Here, we report that mice with a deletion of Slc25a28 (encoding Mfrn2) are born at expected Mendelian ratios, but show decreased male fertility due to reduced sperm numbers and sperm motility. Mfrn2-/- mice placed on a low-iron diet exhibited reduced mitochondrial manganese, cobalt, and zinc levels, but not reduced iron. Hepatocyte-specific loss of Slc25a37 (encoding Mfrn1) in Mfrn2-/- mice did not affect animal viability, but resulted in a 40% reduction in mitochondrial iron and reduced levels of oxidative phosphorylation proteins. Placing animals on a low-iron diet exaggerated the reduction in mitochondrial iron observed in liver-specific Mfrn1/2-knockout animals. Mfrn1-/-/Mfrn2-/- bone marrow-derived macrophages or skin fibroblasts in vitro were unable to proliferate, and overexpression of Mfrn1-GFP or Mfrn2-GFP prevented this proliferation defect. Loss of both mitoferrins in hepatocytes dramatically reduced regeneration in the adult mouse liver, further supporting the notion that both mitoferrins transport iron and that their absence limits proliferative capacity of mammalian cells. We conclude that Mfrn1 and Mfrn2 contribute to mitochondrial iron homeostasis and are required for high-affinity iron import during active proliferation of mammalian cells.
DOI: 10.1074/jbc.RA120.013229
PubMed: 32518166
PubMed Central: PMC7415990
Affiliations:
- France, États-Unis
- Massachusetts, Région Bretagne, Utah, Washington (État), État de New York
- Brest, New York
- Université Columbia
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Paw</name><affiliation wicri:level="2"><nlm:affiliation>Harvard Medical School, Children's Hospital, Boston, Massachusetts, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Harvard Medical School, Children's Hospital, Boston, Massachusetts</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Barasch, Jonathan" sort="Barasch, Jonathan" uniqKey="Barasch J" first="Jonathan" last="Barasch">Jonathan Barasch</name><affiliation wicri:level="4"><nlm:affiliation>Columbia University, New York, New York, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">New York</settlement></placeName><orgName type="university">Université Columbia</orgName></affiliation></author><author><name sortKey="Ward, Diane M" sort="Ward, Diane M" uniqKey="Ward D" first="Diane M" last="Ward">Diane M. Ward</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA diane.mcveyward@path.utah.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32518166</idno><idno type="pmid">32518166</idno><idno type="doi">10.1074/jbc.RA120.013229</idno><idno type="pmc">PMC7415990</idno><idno type="wicri:Area/Main/Corpus">000081</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000081</idno><idno type="wicri:Area/Main/Curation">000081</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000081</idno><idno type="wicri:Area/Main/Exploration">000081</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.</title><author><name sortKey="Seguin, Alexandra" sort="Seguin, Alexandra" uniqKey="Seguin A" first="Alexandra" last="Seguin">Alexandra Seguin</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Jia, Xuan" sort="Jia, Xuan" uniqKey="Jia X" first="Xuan" last="Jia">Xuan Jia</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Earl, Aubree M" sort="Earl, Aubree M" uniqKey="Earl A" first="Aubree M" last="Earl">Aubree M. Earl</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Li, Liangtao" sort="Li, Liangtao" uniqKey="Li L" first="Liangtao" last="Li">Liangtao Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Wallace, Jared" sort="Wallace, Jared" uniqKey="Wallace J" first="Jared" last="Wallace">Jared Wallace</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Qiu, Andong" sort="Qiu, Andong" uniqKey="Qiu A" first="Andong" last="Qiu">Andong Qiu</name><affiliation wicri:level="4"><nlm:affiliation>Columbia University, New York, New York, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">New York</settlement></placeName><orgName type="university">Université Columbia</orgName></affiliation></author><author><name sortKey="Bradley, Thomas" sort="Bradley, Thomas" uniqKey="Bradley T" first="Thomas" last="Bradley">Thomas Bradley</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Shrestha, Rishna" sort="Shrestha, Rishna" uniqKey="Shrestha R" first="Rishna" last="Shrestha">Rishna Shrestha</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Troadec, Marie Berengere" sort="Troadec, Marie Berengere" uniqKey="Troadec M" first="Marie-Bérengère" last="Troadec">Marie-Bérengère Troadec</name><affiliation wicri:level="3"><nlm:affiliation>University Brest, Inserm, EFS, UMR 1078, GGB, F-29200, Brest, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>University Brest, Inserm, EFS, UMR 1078, GGB, F-29200, Brest</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Brest</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>CHRU Brest, Service of Genetics, Laboratory of Chromosome Genetics, Brest, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CHRU Brest, Service of Genetics, Laboratory of Chromosome Genetics, Brest</wicri:regionArea><placeName><region type="region">Région Bretagne</region><region type="old region">Région Bretagne</region><settlement type="city">Brest</settlement></placeName></affiliation></author><author><name sortKey="Hockin, Matt" sort="Hockin, Matt" uniqKey="Hockin M" first="Matt" last="Hockin">Matt Hockin</name><affiliation wicri:level="2"><nlm:affiliation>Department of Human Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Human Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Titen, Simon" sort="Titen, Simon" uniqKey="Titen S" first="Simon" last="Titen">Simon Titen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Human Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Human Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Warner, Dave E" sort="Warner, Dave E" uniqKey="Warner D" first="Dave E" last="Warner">Dave E. Warner</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Dowdle, P Tom" sort="Dowdle, P Tom" uniqKey="Dowdle P" first="P Tom" last="Dowdle">P Tom Dowdle</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Wohlfahrt, Martin E" sort="Wohlfahrt, Martin E" uniqKey="Wohlfahrt M" first="Martin E" last="Wohlfahrt">Martin E. Wohlfahrt</name><affiliation wicri:level="2"><nlm:affiliation>Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region></placeName></affiliation></author><author><name sortKey="Hillas, Elaine" sort="Hillas, Elaine" uniqKey="Hillas E" first="Elaine" last="Hillas">Elaine Hillas</name><affiliation wicri:level="2"><nlm:affiliation>Department of General Surgery, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of General Surgery, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Firpo, Matthew A" sort="Firpo, Matthew A" uniqKey="Firpo M" first="Matthew A" last="Firpo">Matthew A. Firpo</name><affiliation wicri:level="2"><nlm:affiliation>Department of General Surgery, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of General Surgery, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Phillips, John D" sort="Phillips, John D" uniqKey="Phillips J" first="John D" last="Phillips">John D. Phillips</name><affiliation wicri:level="2"><nlm:affiliation>Department of Medicine, University of Utah School of Medicine, Salt Lake City, Utah.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Utah</region></placeName><wicri:cityArea>Department of Medicine, University of Utah School of Medicine, Salt Lake City</wicri:cityArea></affiliation></author><author><name sortKey="Kaplan, Jerry" sort="Kaplan, Jerry" uniqKey="Kaplan J" first="Jerry" last="Kaplan">Jerry Kaplan</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Paw, Barry H" sort="Paw, Barry H" uniqKey="Paw B" first="Barry H" last="Paw">Barry H. Paw</name><affiliation wicri:level="2"><nlm:affiliation>Harvard Medical School, Children's Hospital, Boston, Massachusetts, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Harvard Medical School, Children's Hospital, Boston, Massachusetts</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Barasch, Jonathan" sort="Barasch, Jonathan" uniqKey="Barasch J" first="Jonathan" last="Barasch">Jonathan Barasch</name><affiliation wicri:level="4"><nlm:affiliation>Columbia University, New York, New York, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Columbia University, New York, New York</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">New York</settlement></placeName><orgName type="university">Université Columbia</orgName></affiliation></author><author><name sortKey="Ward, Diane M" sort="Ward, Diane M" uniqKey="Ward D" first="Diane M" last="Ward">Diane M. Ward</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA diane.mcveyward@path.utah.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="eISSN">1083-351X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mitochondrial iron import is essential for iron-sulfur cluster formation and heme biosynthesis. Two nuclear-encoded vertebrate mitochondrial high-affinity iron importers, mitoferrin1 (Mfrn1) and Mfrn2, have been identified in mammals. In mice, the gene encoding Mfrn1, <i>solute carrier family 25 member 37</i> (<i>Slc25a37</i>), is highly expressed in sites of erythropoiesis, and whole-body <i>Slc25a37</i> deletion leads to lethality. Here, we report that mice with a deletion of <i>Slc25a28</i> (encoding Mfrn2) are born at expected Mendelian ratios, but show decreased male fertility due to reduced sperm numbers and sperm motility. <i>Mfrn2</i><sup>-/-</sup> mice placed on a low-iron diet exhibited reduced mitochondrial manganese, cobalt, and zinc levels, but not reduced iron. Hepatocyte-specific loss of <i>Slc25a37</i> (encoding Mfrn1) in <i>Mfrn2</i><sup>-/-</sup> mice did not affect animal viability, but resulted in a 40% reduction in mitochondrial iron and reduced levels of oxidative phosphorylation proteins. Placing animals on a low-iron diet exaggerated the reduction in mitochondrial iron observed in liver-specific <i>Mfrn1/2</i>-knockout animals. <i>Mfrn1</i><sup>-/-</sup>/<i>Mfrn2</i><sup>-/-</sup> bone marrow-derived macrophages or skin fibroblasts <i>in vitro</i> were unable to proliferate, and overexpression of Mfrn1-GFP or Mfrn2-GFP prevented this proliferation defect. Loss of both mitoferrins in hepatocytes dramatically reduced regeneration in the adult mouse liver, further supporting the notion that both mitoferrins transport iron and that their absence limits proliferative capacity of mammalian cells. We conclude that Mfrn1 and Mfrn2 contribute to mitochondrial iron homeostasis and are required for high-affinity iron import during active proliferation of mammalian cells.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32518166</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1083-351X</ISSN><JournalIssue CitedMedium="Internet"><Volume>295</Volume><Issue>32</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>07</Day></PubDate></JournalIssue><Title>The Journal of biological chemistry</Title><ISOAbbreviation>J Biol Chem</ISOAbbreviation></Journal><ArticleTitle>The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.</ArticleTitle><Pagination><MedlinePgn>11002-11020</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1074/jbc.RA120.013229</ELocationID><Abstract><AbstractText>Mitochondrial iron import is essential for iron-sulfur cluster formation and heme biosynthesis. Two nuclear-encoded vertebrate mitochondrial high-affinity iron importers, mitoferrin1 (Mfrn1) and Mfrn2, have been identified in mammals. In mice, the gene encoding Mfrn1, <i>solute carrier family 25 member 37</i> (<i>Slc25a37</i>), is highly expressed in sites of erythropoiesis, and whole-body <i>Slc25a37</i> deletion leads to lethality. Here, we report that mice with a deletion of <i>Slc25a28</i> (encoding Mfrn2) are born at expected Mendelian ratios, but show decreased male fertility due to reduced sperm numbers and sperm motility. <i>Mfrn2</i><sup>-/-</sup> mice placed on a low-iron diet exhibited reduced mitochondrial manganese, cobalt, and zinc levels, but not reduced iron. Hepatocyte-specific loss of <i>Slc25a37</i> (encoding Mfrn1) in <i>Mfrn2</i><sup>-/-</sup> mice did not affect animal viability, but resulted in a 40% reduction in mitochondrial iron and reduced levels of oxidative phosphorylation proteins. Placing animals on a low-iron diet exaggerated the reduction in mitochondrial iron observed in liver-specific <i>Mfrn1/2</i>-knockout animals. <i>Mfrn1</i><sup>-/-</sup>/<i>Mfrn2</i><sup>-/-</sup> bone marrow-derived macrophages or skin fibroblasts <i>in vitro</i> were unable to proliferate, and overexpression of Mfrn1-GFP or Mfrn2-GFP prevented this proliferation defect. Loss of both mitoferrins in hepatocytes dramatically reduced regeneration in the adult mouse liver, further supporting the notion that both mitoferrins transport iron and that their absence limits proliferative capacity of mammalian cells. We conclude that Mfrn1 and Mfrn2 contribute to mitochondrial iron homeostasis and are required for high-affinity iron import during active proliferation of mammalian cells.</AbstractText><CopyrightInformation>© 2020 Seguin et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Seguin</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Xuan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Earl</LastName><ForeName>Aubree M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Liangtao</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wallace</LastName><ForeName>Jared</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Andong</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Columbia University, New York, New York, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bradley</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shrestha</LastName><ForeName>Rishna</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Troadec</LastName><ForeName>Marie-Bérengère</ForeName><Initials>MB</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-2668-9670</Identifier><AffiliationInfo><Affiliation>University Brest, Inserm, EFS, UMR 1078, GGB, F-29200, Brest, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CHRU Brest, Service of Genetics, Laboratory of Chromosome Genetics, Brest, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hockin</LastName><ForeName>Matt</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Human Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Titen</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Human Genetics, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Warner</LastName><ForeName>Dave E</ForeName><Initials>DE</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dowdle</LastName><ForeName>P Tom</ForeName><Initials>PT</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wohlfahrt</LastName><ForeName>Martin E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hillas</LastName><ForeName>Elaine</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of General Surgery, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Firpo</LastName><ForeName>Matthew A</ForeName><Initials>MA</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-7983-3982</Identifier><AffiliationInfo><Affiliation>Department of General Surgery, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Phillips</LastName><ForeName>John D</ForeName><Initials>JD</Initials><AffiliationInfo><Affiliation>Department of Medicine, University of Utah School of Medicine, Salt Lake City, Utah.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaplan</LastName><ForeName>Jerry</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Paw</LastName><ForeName>Barry H</ForeName><Initials>BH</Initials><AffiliationInfo><Affiliation>Harvard Medical School, Children's Hospital, Boston, Massachusetts, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barasch</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Columbia University, New York, New York, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ward</LastName><ForeName>Diane M</ForeName><Initials>DM</Initials><AffiliationInfo><Affiliation>Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA diane.mcveyward@path.utah.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 DK092684</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 DK106829</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P01 HL032262</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 DK070838</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 DK110858</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 DK073462</GrantID><Acronym>DK</Acronym><Agency>NIDDK NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Biol Chem</MedlineTA><NlmUniqueID>2985121R</NlmUniqueID><ISSNLinking>0021-9258</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Slc25a28</Keyword><Keyword MajorTopicYN="N">cell proliferation</Keyword><Keyword MajorTopicYN="N">gene knockout</Keyword><Keyword MajorTopicYN="N">iron metabolism</Keyword><Keyword MajorTopicYN="N">liver</Keyword><Keyword MajorTopicYN="N">membrane transport</Keyword><Keyword MajorTopicYN="N">metal homeostasis</Keyword><Keyword MajorTopicYN="N">mitochondria</Keyword><Keyword MajorTopicYN="N">mitoferrin (Mfrn)</Keyword><Keyword MajorTopicYN="N">solute carrier family 25 member 37 (Slc25a37)</Keyword></KeywordList><CoiStatement>Conflict of interest—The authors declare no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>06</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="pmc-release"><Year>2021</Year><Month>08</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32518166</ArticleId><ArticleId IdType="pii">RA120.013229</ArticleId><ArticleId IdType="doi">10.1074/jbc.RA120.013229</ArticleId><ArticleId IdType="pmc">PMC7415990</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Biol Chem. 2003 Oct 17;278(42):40612-20</Citation><ArticleIdList><ArticleId IdType="pubmed">12902335</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2010 Sep 24;400(3):442-6</Citation><ArticleIdList><ArticleId IdType="pubmed">20807501</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Physiol (Oxf). 2014 Feb;210(2):330-41</Citation><ArticleIdList><ArticleId IdType="pubmed">24034353</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2002 Nov;22(21):7459-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12370293</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2011;2:322</Citation><ArticleIdList><ArticleId IdType="pubmed">21610725</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2009 Feb;29(4):1007-16</Citation><ArticleIdList><ArticleId IdType="pubmed">19075006</ArticleId></ArticleIdList></Reference><Reference><Citation>Oxid Med Cell Longev. 2017;2017:5080128</Citation><ArticleIdList><ArticleId IdType="pubmed">28265337</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2013 Sep 15;454(3):371-86</Citation><ArticleIdList><ArticleId IdType="pubmed">23988125</ArticleId></ArticleIdList></Reference><Reference><Citation>Cytotechnology. 2012 Mar;64(2):187-95</Citation><ArticleIdList><ArticleId IdType="pubmed">22105762</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2009 Jul 15;421(3):463-71</Citation><ArticleIdList><ArticleId IdType="pubmed">19453295</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2011 Nov 15;440(1):137-46</Citation><ArticleIdList><ArticleId IdType="pubmed">21777202</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2014 Nov 28;289(48):33137-48</Citation><ArticleIdList><ArticleId IdType="pubmed">25320081</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2018 Mar 29;9:588</Citation><ArticleIdList><ArticleId IdType="pubmed">29651288</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2002 Jul 5;277(27):24475-83</Citation><ArticleIdList><ArticleId IdType="pubmed">12006577</ArticleId></ArticleIdList></Reference><Reference><Citation>Blood. 2011 May 19;117(20):5494-502</Citation><ArticleIdList><ArticleId IdType="pubmed">21310927</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2013 Oct 1;455(1):57-65</Citation><ArticleIdList><ArticleId IdType="pubmed">23800229</ArticleId></ArticleIdList></Reference><Reference><Citation>Life Sci. 2013 Mar 12;92(4-5):298-304</Citation><ArticleIdList><ArticleId IdType="pubmed">23333832</ArticleId></ArticleIdList></Reference><Reference><Citation>Hepatology. 2006 Feb;43(2 Suppl 1):S45-53</Citation><ArticleIdList><ArticleId IdType="pubmed">16447274</ArticleId></ArticleIdList></Reference><Reference><Citation>Blood. 2004 Feb 15;103(4):1509-14</Citation><ArticleIdList><ArticleId IdType="pubmed">14563638</ArticleId></ArticleIdList></Reference><Reference><Citation>Leukemia. 2019 Jan;33(1):1-14</Citation><ArticleIdList><ArticleId IdType="pubmed">29977016</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Inorg Chem. 2015 Dec;20(8):1229-38</Citation><ArticleIdList><ArticleId IdType="pubmed">26468126</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2004 Aug 6;279(32):33653-61</Citation><ArticleIdList><ArticleId IdType="pubmed">15161905</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2008;3(7):1167-70</Citation><ArticleIdList><ArticleId IdType="pubmed">18600221</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Mar 2;440(7080):96-100</Citation><ArticleIdList><ArticleId IdType="pubmed">16511496</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2017 May 15;36(10):1302-1315</Citation><ArticleIdList><ArticleId IdType="pubmed">28420743</ArticleId></ArticleIdList></Reference><Reference><Citation>Blood. 2008 Aug 1;112(3):866-74</Citation><ArticleIdList><ArticleId IdType="pubmed">18369153</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2011 Jul 1;22(13):2165-74</Citation><ArticleIdList><ArticleId IdType="pubmed">21562223</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2013 Jan 4;288(1):677-86</Citation><ArticleIdList><ArticleId IdType="pubmed">23135267</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Invest. 2014 Oct;124(10):4294-304</Citation><ArticleIdList><ArticleId IdType="pubmed">25157825</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2001 Feb;27(2):181-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11175786</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann N Y Acad Sci. 1966 Jul 14;137(2):643-66</Citation><ArticleIdList><ArticleId IdType="pubmed">4290884</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16263-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19805291</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(1):e29666</Citation><ArticleIdList><ArticleId IdType="pubmed">22253756</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Biol Interact. 2019 May 1;304:73-82</Citation><ArticleIdList><ArticleId IdType="pubmed">30857887</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2018 Mar 9;293(10):3819-3828</Citation><ArticleIdList><ArticleId IdType="pubmed">29305420</ArticleId></ArticleIdList></Reference><Reference><Citation>Free Radic Res. 2015;49(11):1285-95</Citation><ArticleIdList><ArticleId IdType="pubmed">26118715</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Genet. 2008 Aug;24(8):398-407</Citation><ArticleIdList><ArticleId IdType="pubmed">18606475</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2017 May 12;356(6338):608-616</Citation><ArticleIdList><ArticleId IdType="pubmed">28495746</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 1997 Sep;15(9):871-5</Citation><ArticleIdList><ArticleId IdType="pubmed">9306402</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2005 Feb;25(4):1526-36</Citation><ArticleIdList><ArticleId IdType="pubmed">15684401</ArticleId></ArticleIdList></Reference><Reference><Citation>Haematologica. 2018 Aug;103(8):1269-1277</Citation><ArticleIdList><ArticleId IdType="pubmed">29773596</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Rep. 2015 Mar 17;10(10):1681-1691</Citation><ArticleIdList><ArticleId IdType="pubmed">25772356</ArticleId></ArticleIdList></Reference><Reference><Citation>Biometals. 2012 Dec;25(6):1167-75</Citation><ArticleIdList><ArticleId IdType="pubmed">22910858</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1982 Apr 10;257(7):3575-80</Citation><ArticleIdList><ArticleId IdType="pubmed">6460769</ArticleId></ArticleIdList></Reference><Reference><Citation>Genesis. 2009 Dec;47(12):789-92</Citation><ArticleIdList><ArticleId IdType="pubmed">19830819</ArticleId></ArticleIdList></Reference><Reference><Citation>Blood. 2019 Jun 6;133(23):2507-2517</Citation><ArticleIdList><ArticleId IdType="pubmed">30952671</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Metab. 2019 Jan;1(1):158-171</Citation><ArticleIdList><ArticleId IdType="pubmed">31106291</ArticleId></ArticleIdList></Reference><Reference><Citation>J Inherit Metab Dis. 2014 Jul;37(4):565-75</Citation><ArticleIdList><ArticleId IdType="pubmed">24797559</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Massachusetts</li><li>Région Bretagne</li><li>Utah</li><li>Washington (État)</li><li>État de New York</li></region><settlement><li>Brest</li><li>New York</li></settlement><orgName><li>Université Columbia</li></orgName></list><tree><country name="États-Unis"><region name="Utah"><name sortKey="Seguin, Alexandra" sort="Seguin, Alexandra" uniqKey="Seguin A" first="Alexandra" last="Seguin">Alexandra Seguin</name></region><name sortKey="Barasch, Jonathan" sort="Barasch, Jonathan" uniqKey="Barasch J" first="Jonathan" last="Barasch">Jonathan Barasch</name><name sortKey="Bradley, Thomas" sort="Bradley, Thomas" uniqKey="Bradley T" first="Thomas" last="Bradley">Thomas Bradley</name><name sortKey="Dowdle, P Tom" sort="Dowdle, P Tom" uniqKey="Dowdle P" first="P Tom" last="Dowdle">P Tom Dowdle</name><name sortKey="Earl, Aubree M" sort="Earl, Aubree M" uniqKey="Earl A" first="Aubree M" last="Earl">Aubree M. Earl</name><name sortKey="Firpo, Matthew A" sort="Firpo, Matthew A" uniqKey="Firpo M" first="Matthew A" last="Firpo">Matthew A. Firpo</name><name sortKey="Hillas, Elaine" sort="Hillas, Elaine" uniqKey="Hillas E" first="Elaine" last="Hillas">Elaine Hillas</name><name sortKey="Hockin, Matt" sort="Hockin, Matt" uniqKey="Hockin M" first="Matt" last="Hockin">Matt Hockin</name><name sortKey="Jia, Xuan" sort="Jia, Xuan" uniqKey="Jia X" first="Xuan" last="Jia">Xuan Jia</name><name sortKey="Kaplan, Jerry" sort="Kaplan, Jerry" uniqKey="Kaplan J" first="Jerry" last="Kaplan">Jerry Kaplan</name><name sortKey="Li, Liangtao" sort="Li, Liangtao" uniqKey="Li L" first="Liangtao" last="Li">Liangtao Li</name><name sortKey="Paw, Barry H" sort="Paw, Barry H" uniqKey="Paw B" first="Barry H" last="Paw">Barry H. Paw</name><name sortKey="Phillips, John D" sort="Phillips, John D" uniqKey="Phillips J" first="John D" last="Phillips">John D. Phillips</name><name sortKey="Qiu, Andong" sort="Qiu, Andong" uniqKey="Qiu A" first="Andong" last="Qiu">Andong Qiu</name><name sortKey="Shrestha, Rishna" sort="Shrestha, Rishna" uniqKey="Shrestha R" first="Rishna" last="Shrestha">Rishna Shrestha</name><name sortKey="Titen, Simon" sort="Titen, Simon" uniqKey="Titen S" first="Simon" last="Titen">Simon Titen</name><name sortKey="Wallace, Jared" sort="Wallace, Jared" uniqKey="Wallace J" first="Jared" last="Wallace">Jared Wallace</name><name sortKey="Ward, Diane M" sort="Ward, Diane M" uniqKey="Ward D" first="Diane M" last="Ward">Diane M. Ward</name><name sortKey="Warner, Dave E" sort="Warner, Dave E" uniqKey="Warner D" first="Dave E" last="Warner">Dave E. Warner</name><name sortKey="Wohlfahrt, Martin E" sort="Wohlfahrt, Martin E" uniqKey="Wohlfahrt M" first="Martin E" last="Wohlfahrt">Martin E. Wohlfahrt</name></country><country name="France"><region name="Région Bretagne"><name sortKey="Troadec, Marie Berengere" sort="Troadec, Marie Berengere" uniqKey="Troadec M" first="Marie-Bérengère" last="Troadec">Marie-Bérengère Troadec</name></region><name sortKey="Troadec, Marie Berengere" sort="Troadec, Marie Berengere" uniqKey="Troadec M" first="Marie-Bérengère" last="Troadec">Marie-Bérengère Troadec</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= The mitochondrial metal transporters mitoferrin1 and mitoferrin2 are required for liver regeneration and cell proliferation in mice.
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